Is sample dentaturation above 95°C, the golden standard?-标准-资讯-生物在线
·

Is sample dentaturation above 95°C, the golden standard?

作者：Agrisera AB 2020-10-21T00:00 (访问量:2469)

 Is sample dentaturation above 95°C, the golden standard? 

The temperature of sample denaturation may influence the detected band pattern in Western blot. We recommend:

70°C for 5-10 minutes for total cell extracts from various plant tissues and algal cells. Higher denaturation temperatures may lead to protein aggregation, followed by lack of a specific band signal in Western blot. 

Special care should be taken in case of membrane proteins, like H+ATPase, which may aggregate in high temperatures and thus never enter the gel. 

Therefore, we recommend to carefully study instructions provided on the product information sheet for a given antibody. 

Some antibodies may mostly work on proteins in native conformation and will therefore be suitable to use in techniques like ChIP and immunoprecipitation, while other antibodies will recognize mostly epitopes burried inside a protein and exposed by sample denaturation. Such details are important to pay attention to when chosing an antibody suitable for your experiments. 

  
Western blot detection
2020-07-27 @ 19:50:27
Agrisera AB 商家主页

地 址： 21 Vännäs, SWEDEN, Europe

联系人： Dr. Joanna Porankiewicz-Asplund

电 话： 0046704137107

传 真：

Email：61382170@qq.com

相关咨询

Antigen, immunogen and epitope, what is the difference? (2022-05-24T10:12 浏览数:4625)

How to transfer proteins of low molecular weight? (2022-05-24T10:11 浏览数:4771)

How to save time and shorten Western blot protocol? (2022-05-24T10:09 浏览数:3129)

How to get an intense signal in Western blot using a weak antibody? (2022-04-14T17:01 浏览数:7148)

Antigen, immunogen and epitope, what is the difference? (2022-04-14T17:00 浏览数:6901)

What are the benefits of using directly conjugated primary antibodies? (2022-04-14T16:59 浏览数:5424)

How to transfer proteins of low molecular weight? (2022-04-14T16:57 浏览数:4728)

20% discount on loading control and compartment marker antibodies (2022-04-14T16:57 浏览数:4757)

New epitope tag antibody mNeonGreen, detects mNG tag expressed in plant and algal vectors (2022-02-23T10:28 浏览数:779)

New epitope tag antibody mNeonGreen, detects mNG tag expressed in plant and algal vectors (2022-02-23T10:28 浏览数:4066)

ADVERTISEMENT