| Can tubulin be used as a loading control in metal stress? To check this Anti-tubulin alpha chain antibodies were used on extracts from: Nicotiana tabacum plants were treated with silver nanoparticles (AgNPs) with 2 different coatings. Tubulin alpha protein content marked by a blue arrow in the image to the right, was influenced by silver nanoparticle treatment. Similar results were obtained for tubulin gamma chain. Therefore, it can be concluded that: Metal induced stress affected the expression of tubulin alpha and gamma, and that therefore, these proteins cannot be used as loading control under these conditions. |  All experimental details can be found here. Courtesy of MSc, Karla Košpić, University of Zagreb, Faculty of Science Department of Biology, Croatia |
Tubulin alpha and gamma are not suitable as a loading control for metal stress
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