肌原纤蛋白2(FBN2)重组蛋白

YB368Hu014
Recombinant Fibrillin 2 (FBN2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
肌原纤蛋白2(FBN2)重组蛋白[ PROPERTIES ]
Residues: Thr1550~Cys1791 (Accession # P35556),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Location: Secreted, extracellular
space, extracellular matrix.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
肌原纤蛋白2(FBN2)重组蛋白Predicted isoelectric point: 5.7
Predicted Molecular Mass: 58.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
肌原纤蛋白2(FBN2)重组蛋白than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGS-T PGRYECNCPP
DFQ L NP T GV G CV D N RV G N CY LK F GP R G D G S LS C N T EI GV G VS RS S C C C S L
GKAWGNPCET CPPVNSTEYY TLCPGGEGFR PNPITIILED IDECQELPGL CQGGNCINTF
GSFQCECPQG YYLSEDTRIC EDIDECFAHP GVCGPGTCYN TLGNYTCICP PEYMQVNGGH
NCMDMRKSF C YRSY NGT T CE NE LPFNVTK R MCCCTY NVGK AWNKP CEP CP
TPGTADFKTI C