N-乙酰β-D-氨基葡萄糖苷酶(NAGase)重组蛋白

YB069Ra021
N-Acetyl Beta-D-Glucosaminidase (NAGase)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
N-乙酰β-D-氨基葡萄糖苷酶(NAGase)重组蛋白[ PROPERTIES ]
Residues: Glu319~Thr450 (Accession # O60502),
with N-terminal His-Tag.
Host: E. coli
Subcellular Location: Cytoplasm. Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted isoelectric point: 5.0
N-乙酰β-D-氨基葡萄糖苷酶(NAGase)重组蛋白Predicted Molecular Mass: 15.8kDa
Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
N-乙酰β-D-氨基葡萄糖苷酶(NAGase)重组蛋白[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF- EA NYVAIHTLAT WYKSNMNGVR KDVVMTDSED STVSIQIKLE
NEGSDEDIET DVLYSPQMAL KLALTEWLQE FGVPHQYSSR QVAHSGAKAS VVDGTPLVAA
PSLNATTVVT TVYQEPIMSQ GAALSGEPTT
[ REFERENCES ]
1. Heckel D., et al. (1998) Hum. Mol. Genet. 7:1859-1872. 2. Comtesse N., et al. (2001) Biochem. Biophys. Res. Commun. 283:634-640. 3. Gao Y., et al. (2001) J. Biol. Chem. 276:9838-9845. 4. Wells L., et al. (2002) J. Biol. Chem. 277:1755-1761.