分子量:28.2 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human PD-L1, Strep Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its binding ability in a functional ELISA.Immobilized Human PD-L1, Strep Tag (Cat# PD1-H5282) at 5 μg/mL (100 μl/well) can bind Biotinylated Human PD-1, His Tag & Fc Tag (Cat# PD1-H82F2 ) .

产品特性:Human PD-L1, Strep Tag is fused with twin strep tag at the C-terminus, and has a calculated MW of 28.2 kDa. The predicted N-terminus is Phe 19. The reducing (R) protein migrates as 35-42 kDa in SDS-PAGE  due to glycosylation.

产品背景:Programmed cell death 1 ligand 1 (PD-L1) is also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1), is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses. B7-H1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain. PD-L1 is highly expressed in the heart, skeletal muscle, placenta and lung and weakly expressed in the thymus, spleen, kidney and liver. PD-L1 is expressed on activated T-cells, B-cells, dendritic cells, keratinocytes and monocytes. PD-L1 is up-regulated on T- and B-cells, dendritic cells, keratinocytes and monocytes after LPS and IFNG activation and up-regulated in B-cells activated by surface Ig cross-linking. PD-L1 involve in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner.