Overview

VitroGel® MSC is a xeno-free (animal origin-free) hydrogel system developed to support three-dimensional (3D) cultures of mesenchymal stem cells (MSCs).  This hydrogel system can be used to make hydrogel cell beads for MSC scale-up.  Microcarriers are not required for MSC scale-up.

VitroGel MSC is ready-to-use with an optimized formulation that fully supports the rapid expansion of MSCs. Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D culture or hydrogel-cell bead generation. This hydrogel system is compatible with most MSC culture media and tissue culture vessels. By using the VitroGel Cell Recovery Solution, the cell harvesting after 3D culture is simple and effective.

3D cell culture process in 20 min – “Just add cells”

VitroGel MSC is ready-to-use. Just mix with your cells.

MSC 3D scale-up process in 20 min – Hydrogel-cell bead formation

VitroGel MSC is ready-to-use. Just mix with your cells and add as droplets.

Specifications

Formulation	Xeno-free, functional hydrogel
Use	3D cell culture, 2D hydrogel coating, hydrogel-cell bead formation
Operation	Ready-to-use at room temperature
Biocompatibility	Biocompatible, safe for animal studies
Injection	Injectable hydrogel for in vivo studies and lab automation
Cell Harvesting	Easy cell recovery using VitroGel Cell Recovery Solution
pH	Neutral
Storage	Store at 2-8°C. Ships at ambient temperature
Sizes	10 mL and 2 mL
Usage	300 uses (10 mL), 60 uses (2mL)

Data and References

Handbooks and Resources

Product Documentation

• Protocol

• Cell Recovery from Gel

• Cell Fluorescent Staining in Gel for Nuclei and Actin Filament

• Cell Lysis for Downstream Analysis

• Cell Viability Live-Dead Assay Protocol

• Immunofluorescence Staining

• Sample Preparation for Histological Analysis