重组人CD16A蛋白（Recombinant Human CD16A Protein），GMP级

产品货号：GMP-TL686

产品描述（Description）

CD16 is a low affinity Fc receptor, and has been identified as Fc receptors FcγRIIIa (CD16a) and FcγRIIIb (CD16b). These receptors bind to the Fc portion of IgG antibodies. CD16 encoded by two different highly homologous genes in a cell type-specific manner.CD16 is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. CD16a antigen is also known as Low affinity immunoglobulin gamma Fc region receptor III-A, Fc-gamma RIII-alpha. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed natural killer cells, macrophages, subpopulation of T-cells, immature thymocytes and placentaltrophoblasts.CD16a is involved in phagocytosis, secretion of enzymes and inflammatory mediators, antibody-dependent cytotoxicity and clearance of immune complexes. Aberrant expression or mutations of CD16a is implicated in susceptibility to recurrent viral infections, systemic lupus erythematosus, and alloimmune neonatal neutropenia.

分子别名（Synonym）

FCGR3A、CD16A、FCG3、FCGR3、IGFR3

表达宿主（Expression host）

HEK293细胞

来源（Source）

Human

蛋白序列（Protein Sequence）

DNA 序列编码人 CD16a (F176)（UniProtKB/Swiss-Prot: P08637.2）表达带有 His 标签在 C 末端

分子量（Molecular Weight）

重组人 CD16a (F176)蛋白 包含 254 个氨基酸，预测理论分子量为 23.5 kD

纯度（Purity）

≥95%采用SDS-PAGE凝胶和高效液相色谱分析

内毒素（Endotoxin）

＜0.1EU/ug

生物活性（Biological Activity）

固定化 Anti-CD16a antibody 1ug/mL(100uL/孔)可以结合人 CD16a (F176) (His 标签)的线性范围为 1-20ng/mL

稳定性（Stability）

冻干的样本的可以在4℃保存24个月，溶解后的液体可以于-20℃保存6-12个月，并且避免反复冻融

组成（Component）

含有6%甘露醇的无菌PBS（PH 7.4）的冻干粉

参考文献

1、 Stimulation of Fc gamma RIIIA results in phospholipase C-gamma 1 tyrosine phosphorylation and p56lck activation J Exp Med. 1992 Dec 1; 176(6): 1745–1750.

2、 Phosphatidylinositol-3 kinase activation induced upon Fc gamma RIIIA- ligand interaction J Exp Med. 1994 Feb 1; 179(2): 551–558.

3、Antibody fucosylation lowers FcγRIIIa/CD16a affinity by limiting the conformations sampled by the N162-glycan Daniel J. Falconer, Ganesh P. Subedi, Aaron M. Marcella, Adam W. Barb ACS Chem Biol. 2018 Aug 17; 13(8): 2179–2189. Published online 2018 Jul 27. doi: 10.1021/acschembio.8b00342

注意：For research and manufacturing use.