form:powder.
technique(s):cell culture | mammalian: suitable.
components:phenol red: no;HEPES: no;sodium pyruvate: no;L-glutamine: no;NaHCO3: no;Earle’s salts (5% CO2): yes.
General description:Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.