RNF88/TRIM5 alpha,环指蛋白88抗体

产品编号HZ-9153R
英文名称RNF88/TRIM5 alpha
中文名称环指蛋白88抗体
别 名TRIM5 alpha; RING finger protein 88; RNF88; TRIM5; TRIM5_HUMAN; tripartite motif protein 5; tripartite motif protein TRIM5; Tripartite motif-containing protein 5.
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human,
RNF88/TRIM5 alpha,环指蛋白88抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量56kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human TRIM5α/RNF88 corresponding to amino acids 351-450.
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈.
RNF88/TRIM5 alpha,环指蛋白88抗体PubMedPubMed
产品介绍background:
TRIM5a is a 493 amino acid member of the large tripartite motif protein (TRIM) family. TRIM proteins are composed of three zinc-binding domains, a RING, a B-box 2 and a coiled-coil domain, and they use homomultimerization to identify different cell compartments. Some TRIM proteins, such as TRIM5? also possess a carboxy-terminal B30.2 (SPRY) domain and localize to the cytoplasm. TRIM5?mediates innate intracellular retroviral resistance, which is dependent on its carboxy-terminal domain. The three variable regions of the B30.2 domain form loops on one side of the B30.2 core structure of TRIM5?which may form a binding surface for the virus. TRIM5?trimerization plays a major role in its affinity for the retroviral capsid, and in its ability to inhibit virus infection. The linker region between the coiled-coil and B30.2 domains of TRIM5?is required for this trimerization. TRIM5?blocks infection after the virus has entered the cell.

Function:
Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.

RNF88/TRIM5 alpha,环指蛋白88抗体Subunit:
Isoform Alpha forms homotrimers, and may interact with retroviral CA protein. Isoform Delta interacts with BTBD1 and BTBD2.

Subcellular Location:
Cytoplasm, P-body.

Post-translational modifications:
Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).

Similarity:
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.

Gene ID:
85363

Database links:
Entrez Gene: 85363 Human
Omim: 608487 Human
SwissProt: Q9C035 Human
Unigene: 370515 Human

RNF88/TRIM5 alpha,环指蛋白88抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

科学家经过研究发现TRIM5蛋白有阻止HIV病毒繁殖的作用，在艾滋病毒感染过程中，一旦HIV病毒进入细胞，TRIM5蛋白立刻拦截并阻止其繁殖。因此， TRIM5是先天免疫系统中对HIV病毒的感应器。与适应性免疫系统“后发制人”不同，先天免疫系统能够在接触到病原体时立刻将其消除。

产品图片

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-TRIM5α/RNF88 Polyclonal Antibody, Unconjugated(HZ-9153R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining