周期素依赖性激酶抑制因子2A(CDKN2A)重组蛋白

YB794Ra011
Recombinant Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Ser8~Asp156 (Accession # P42771),
周期素依赖性激酶抑制因子2A(CDKN2A)重组蛋白with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Cytoplasm. Nucleus.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 6.4
Predicted Molecular Mass: 19.7kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
周期素依赖性激酶抑制因子2A(CDKN2A)重组蛋白Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
周期素依赖性激酶抑制因子2A(CDKN2A)重组蛋白sequence is listed below.
MGSSHHHHHH SSGLVP RGSH MASMTGGQQM GRGSEF - SME PSADWLATAA
ARGRVEEVRA LLEAGALPNA PNSYGRRPIQ VMMMGSARVA ELLLLHGAEP NCADPATLTR
PVHDAAREGF LDTLVVLHRA GARLDVRDAW GRLPVDLAEE LGHRDVARYL RAAAGGTRGS
NHARIDAAEG PSDIPD
[ REFERENCES ]
1. Serrano M., et al. (1993) Nature 366:704-707. 2. Kitagawa Y., et al. (2002) J. Biol. Chem. 277:46289-46297. 3. Lin Y.C., et al. (2007) Oncogene 26:7017-7027. 4. Hara E., et al. (1996) Mol. Cell. Biol. 16:859-867.