GBF1,蛋白转运抑制剂GBF1抗体

产品编号HZ-11266R
英文名称GBF1
中文名称蛋白转运抑制剂GBF1抗体
别 名ARF1GEF; BFA resistant GEF 1; BFA-resistant GEF 1; FLJ21263; FLJ21500; GBF1; GBF1_HUMAN; Golgi specific brefeldin A resistance factor 1; Golgi specific Brefeldin A-resistance factor; Golgi-specific brefeldin A-resistance guanine nucleotide exchange; Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1; KIAA0248; MGC134877; MGC134878.
说 明 书0.1ml 0.2ml
研究领域细胞生物 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep,
GBF1,蛋白转运抑制剂GBF1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量206kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Brefeldin A
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
GBF1,蛋白转运抑制剂GBF1抗体PubMedPubMed
产品介绍background:
Protein trafficking to the membrane requires formation of coated carrier vesicles, such as COPI-coated vesicles from the cis-golgi, a process triggered by membrane binding of the GTP-bound form of ADP-ribosylation factors. Normally, brefeldin A (BFA) blocks this action by inhibiting guanine nucleotide exchange factors (GEFs) for ADP-ribosylation factor. However, GBF1, a member of the sec7-domain family of GEFs, allows cells to maintain normal golgi morphology and grow in the presence of BFA. The gene encoding the human GBF1 protein maps to chromosome 10q24, with the sec7-domain centrally positioned, and a proline-rich C-terminus. Based on mutatagenesis analysis, this proline rich region appears to interact with p115 in a functionally significant manner.

Function:
Promotes guanine-nucleotide exchange on ARF5. Promotes the activation of ARF5 through replacement of GDP with GTP.

Tissue Specificity:
Ubiquitous.

Similarity:
Contains 1 SEC7 domain.

Database links:
Entrez Gene: 8729 Human
Entrez Gene: 107338 Mouse
Omim: 603698 Human
SwissProt: Q92538 Human
Unigene: 290243 Human
Unigene: 271620 Mouse

GBF1,蛋白转运抑制剂GBF1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-GBF1 Polyclonal Antibody, Unconjugated(HZ-11266R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining