BstH2I
产品名称： BstH2I
英文名称： BstH2I
产品编号： HZ600282
产品价格： 0
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： null
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749 点击查看
- 传真 : 点击查看
- 邮箱 : www.shzbio.net
- 二维码 : 点击查看
BstH2I
产品描述
使用说明
高浓度的酶活性，may result in to obtain 100%明星活动，牛血清白蛋白（BSA）should be added to the反应混合到最后1×100μg／ml浓度的BSA。do not use for long培养。
技术规格
Unit One Unit of the definition酶水解is required to the amount ofλDNA 1μg in at 1小时150度总量在50μl反应。
网站rgcgc↑y recognition
嗜热脂肪芽孢杆菌H2的来源
我们assayedλDNA
优化的缓冲区（33毫米和醋酸（pH 7.9毫米（10°C）；醋酸醋酸钾镁；66毫米1毫米；+ BSA DTT。）
温度150度优化
10毫米KH2PO4的存储条件（pH 7.2）；EDTA，100 mM NaCl 0.1毫米；7毫米；2 -巯基乙醇200μg／ml；BSA；50%的甘油。商店在零下20摄氏度。
在20 ligations折叠酶overdigestion with more than 90% of the and can be重新ligated DN**段。
非特异性水解后没有检测到孵化nonspecific activity was withλDNA 1μg of of of hours for 16 20 UA听说是八周的酶在150度。
甲基化效应不灵敏的测试
热灭活的酶灭活°C is at by潜伏期为20分钟。
在campatible bfoi，bsp143ii，haeii
使用说明
High enzyme concentration may result in star activity To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl. |
| Recognition sites | RGCGC↑Y |
| Source | Bacillus stearothermophilus H2 |
| Assayed on | Lambda DNA |
| Optimal buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA |
| Optimal temperature | 65°C |
| Storage conditions | 10 mM KH2PO4(pH 7.2); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C. |
| Ligations | After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80°C for 20 mins. |
| Campatible Ends | BfoI, Bsp143II, HaeII |