ENSA,α内磺肽抗体

产品编号HZ-11793R
英文名称ENSA
中文名称α内磺肽抗体
别 名Alpha endosulfine; ARPP 19e; Endosulfine alpha; ENSA_HUMAN; Alpha-endosulfine; ARPP-19e.
说 明 书0.1ml 0.2ml
研究领域神经生物学 生长因子和激素 通道蛋白 细胞膜受体
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Dog, Cow, Horse, Rabbit,
ENSA,α内磺肽抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量13kDa
细胞定位细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ENSA/ARPP-19
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ENSA,α内磺肽抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene belongs to a highly conserved cAMP-regulated phosphoprotein (ARPP) family. This protein was identified as an endogenous ligand for the sulfonylurea receptor, ABCC8/SUR1. ABCC8 is the regulatory subunit of the ATP-sensitive potassium (KATP) channel, which is located on the plasma membrane of pancreatic beta cells and plays a key role in the control of insulin release from pancreatic beta cells. This protein is thought to be an endogenous regulator of KATP channels. In vitro studies have demonstrated that this protein modulates insulin secretion through the interaction with KATP channel, and this gene has been proposed as a candidate gene for type 2 diabetes. At least eight alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Function:
ENSA (Alpha endosulfine) is expressed in a wide range of tissues including muscle, brain, and endocrine tissues. The recombinant protein inhibits binding of labeled glibenclamide to beta cell membranes. It also inhibits cloned K(ATP) channel currents and thereby stimulates insulin secretion. It was proposed that endosulfine is an endogenous regulator of the K(ATP) channel, which has a key role in the control of insulin release and, more generally, couples cell metabolism to electrical activity.

Subunit:
Interacts (when phosphorylated at Ser-67) with PPP2R2D. Interacts with ABCC8. Interacts with SNCA; interaction is disrupted when phosphorylated at Ser-109.

ENSA,α内磺肽抗体Subcellular Location:
Cytoplasmic

Tissue Specificity:
Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas.

Post-translational modifications:
Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA.

DISEASE:

Similarity:
Belongs to the endosulfine family.

Database links:
Entrez Gene: 281142 Cow
Entrez Gene: 2029 Human
Entrez Gene: 56205 Mouse
Entrez Gene: 60334 Rat
Omim: 603061 Human
SwissProt: O43768 Human
SwissProt: P60840 Mouse
SwissProt: P60841 Rat
Unigene: 632456 Human
Unigene: 27154 Rat

ENSA,α内磺肽抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.