尿嘧啶磷酸核糖转移酶(UPRT)重组蛋白

YB983Hu013
Uracil Phosphoribosyltransferase Phosphoribosyltransferase Phosphoribosyltransferase Phosphoribosyltransferase (UPRT)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
尿嘧啶磷酸核糖转移酶(UPRT)重组蛋白[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Asn75~Ala297 (Accession # Q96BW1),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Cytoplasm. Nucleus.
Purity: Purity: Purity: Purity: >95%
尿嘧啶磷酸核糖转移酶(UPRT)重组蛋白Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.2
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 51.8kDa
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
尿嘧啶磷酸核糖转移酶(UPRT)重组蛋白[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL
DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD
VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD
GS T S G S G H H H HH HSA G LVP R GS TA IG MK E T AA AK F E R Q HM DSP D L G T L EV
LFQGPLGSEF-NSEGNS GSGDSSSYDA PAGNSFLEDC ELSRQIGAQL KLLPMNDQIR
ELQTIIRDKT ASRGDFMFSA DRLIRLVVEE GLNQLPYKEC MVTTPTGYKY EGVKFEKGNC
GVSIMRSGEA MEQGLRDCCR SIRIGKILIQ SDEETQRAKV YYAKFPPDIY RRKVLLMYPI
LSTGNTVIEA VKVLIEHGVQ PSVIILLSLF STPHGAKSII QEFPEITILT TEVHPVA