ACHE,乙酰胆碱酯酶抗体

产品编号HZ-2511R
英文名称ACHE
中文名称乙酰胆碱酯酶抗体
别 名Acetylcholinesterase; Acetylcholine acetylhydrolase; Acetylcholinesterase YT blood group; ACHE; ACHE protein; Apoptosis related acetylcholinesterase; ARACHE; N ACHE; N-ACHE; YT; Yt blood group; ACES_HUMAN.
规格价格0.1ml 0.2ml 
说 明 书0.1ml 0.2ml
研究领域神经生物学 信号转导
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Cow, Horse,
ACHE,乙酰胆碱酯酶抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量68kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human AchE
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ACHE,乙酰胆碱酯酶抗体PubMedPubMed
产品介绍background:
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Function:
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.

Subunit:
Interacts with PRIMA1. The interaction with PRIMA1 is required to anchor it to the basal lamina of cells and organize into tetramers. Isoform H generates GPI-anchored dimers; disulfide linked. Isoform T generates multiple structures, ranging from monomers and dimers to collagen-tailed and hydrophobic-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, isoform T subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers, from monomers and dimers. Isoform R may be monomeric.

ACHE,乙酰胆碱酯酶抗体Subcellular Location:
Cell junction, synapse. Secreted. Cell membrane; Peripheral membrane protein.
Isoform T: Nucleus. Note=Only observed in apoptotic nuclei.
Isoform H: Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.

Tissue Specificity:
Isoform H is highly expressed in erythrocytes.

Similarity:
Belongs to the type-B carboxylesterase/lipase family.

Gene ID:
43

Database links:
Entrez Gene: 540446 Cow
Entrez Gene: 43 Human
Entrez Gene: 11423 Mouse
Entrez Gene: 83817 Rat
Omim: 100740 Human
SwissProt: O62763 Cat
SwissProt: P23795 Cow
SwissProt: P22303 Human
SwissProt: P21836 Mouse
SwissProt: P37136 Rat
Unigene: 154495 Human
Unigene: 255464 Mouse
Unigene: 105879 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Tissue/cell: rat heart tissue;4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ACHE Polyclonal Antibody, Unconjugated(bs-2511R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.