Connexin-43抗体-第二抗体-抗体-生物在线

产品名称： Connexin-43抗体

英文名称： Connexin-43Antibody

产品编号： XY 15386-1-

产品价格： null

产品产地：中国/美国

品牌商标： XYbscience

更新时间： 2023-08-17T09:55:27

使用范围： WB,ELISA,IHC-P,IHC-F,IF

上海信裕生物科技有限公司

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CONNEXIN-43抗体

Applications

In-house applications	ELISA, WB, IHC, FC, IP Application not tested? Please see our Guarantee.
Published applications	IF	Publications	2 citations –view here
Species specificity	Human,Mouse,Rat; other species not tested.
Published Species	Mouse
Images Prev1 2NextView more validation data Immunohistochemical of paraffin-embedded mouse heart using 15386-1-AP(GJA1 antibody) at dilution of 1:50 (under 10x lens) Immunohistochemical of paraffin-embedded mouse heart using 15386-1-AP(GJA1 antibody) at dilution of 1:50 (under 40x lens) 1X10^6 Jurkat cells were stained with 0.5ug Connexin 43 antibody (15386-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. HeLa cells were subjected to SDS PAGE followed by western blot with 15386-1-AP(GJA1 antibody) at dilution of 1:500 IP Result of anti-Connexin 43 (IP:15386-1-AP, 4ug; Detection:15386-1-AP 1:500) with mouse heart tissue lysate 3200ug.
Positive WB detected in	HeLa cells, Jurkat cells, K-562 cells
Positive IP detected in	Mouse heart tissue
Positive IHC detected in	Mouse heart tissue, human heart tissue
Positive FC detected in	Jurkat cells
Recommended dilution	WB : 1:500-1:5000 IP : 1:200-1:2000 IHC : 1:20-1:200

CONNEXIN-43抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation

Product Information

Source	Rabbit	Purification method	Antigen affinity purification
Isotype	IgG	Storage	PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20^oC.

Immunogen Information CONNEXIN-43抗体

Immunogen	Connexin-43 fusion protein ag6698	Full name	Gap junction protein, alpha 1, 43kDa
Calculated molecular weight	43 kDa	Observed molecular weight	47kd
GenBank accession number	BC026329	Gene ID (NCBI)	2697
Gene symbol	GJA1	Synonyms	Connexin-43, CX43, DFNB38, Gap junction alpha 1 protein, GJA1, GJAL, ODDD CONNEXIN-43抗体

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