CD33,CD33抗体

产品编号HZ-2042R
英文名称CD33
中文名称CD33抗体
别 名CD33_HUMAN; CD33 antigen; CD33 molecule; FLJ00391; gp67; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; p67; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; SIGLEC3; Siglec-3.
说 明 书0.1ml 0.2ml
研究领域免疫学 神经生物学 干细胞 细胞类型标志物
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human,
CD33,CD33抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:50-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量40/25kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CD33
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CD33,CD33抗体PubMedPubMed
产品介绍background:
CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.

Function:
Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).

Subunit:
Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation.

Subcellular Location:
Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:
Monocytic/myeloid lineage cells.

Post-translational modifications:
Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11. Phosphorylation of Tyr-358 is involved in binding to PTPN6.

Similarity:
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Gene ID:
945

CD33,CD33抗体Database links:
Entrez Gene: 945 Human
Entrez Gene: 12489 Mouse
Entrez Gene: 690492 Rat
Omim: 159590 Human
SwissProt: P20138 Human
SwissProt: Q63994 Mouse
Unigene: 83731 Human
Unigene: 140157 Mouse

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

CD33(Siglec-3)也是急性淋巴细胞白血病抗原,细胞表面跨膜糖蛋白.CD33表达于早期B淋巴细胞,某些粒性白细胞,骨髓基质细胞,部分上皮组织及其起源的肿瘤细胞中也有表达.主要应用于某些恶性淋巴瘤和白血病的分型.