E2F2,转录因子E2F-2抗体

产品编号HZ-1001R
英文名称E2F2
中文名称转录因子E2F-2抗体
别 名dE2F2; E2F-2; E2F2; E2F2_HUMAN; Transcription factor E2F2; E2F transcription factor 2.
说 明 书0.1ml 0.2ml
研究领域免疫学 染色质和核信号 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Horse,
E2F2,转录因子E2F-2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量48kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human E2F2 N-terminus
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
E2F2,转录因子E2F-2抗体PubMedPubMed
产品介绍background:
E2F/DP family winged-helix DNA-binding domain. This family contains the transcription factor E2F and its dimerisation partners TDP1 and TDP2, which stimulate E2F-dependent transcription. E2F binds to DNA as a homodimer or as a heterodimer in association with TDP1/2, the heterodimer having increased binding efficiency. The crystal structure of an E2F4-DP2-DNA complex shows that the DNA-binding domains of the E2F and DP proteins both have a fold related to the winged-helix DNA-binding motif. Recognition of the central c/gGCGCg/c sequence of the consensus DNA-binding site is symmetric, and amino acids that contact these bases are conserved among all known E2F and DP proteins.

Function:
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.

Subunit:
Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F2 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB1, thus releasing the active complex. Binds EAPP.

Subcellular Location:
Nucleus.

E2F2,转录因子E2F-2抗体Tissue Specificity:
Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.

Post-translational modifications:
Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase (By similarity).

Similarity:
Belongs to the E2F/DP family.

Gene ID:
1870

Database links:
Entrez Gene: 1870 Human
Entrez Gene: 242705 Mouse
Omim: 600426 Human
SwissProt: Q14209 Human
SwissProt: P56931 Mouse
Unigene: 194333 Human
Unigene: 307932 Mouse

E2F2,转录因子E2F-2抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

转录调节因子（Transcriptin Regulators）
E2F2(又称：TFE-25)—属于调节性转录因子E2F家族。