Description Passive elution and electro elution are the most common methods for extracting proteins and/or nucleic acid from polyacrylamide/agarose gels. Protein extraction by passive elution usually takes overnight incubation. Eluted proteins are significantly diluted and require further concentration. Protein extraction by electro elution is faster (30 min to 2 h) than passive elution but it requires a special elution device that works less effectively for larger proteins (>70 Kda). The electro buffer usually contains detergent and other chemicals at a concentration that may interfere with downstream applications. Here we feature a rapid and instrument-free protein/nucleic acid extraction kit. Proteins/nucleic acid can be extracted from gels in <10 min with high yield. The elution buffer can be a detergent-containing buffer or pure water depending upon methods of gel staining. The elution volume is between 10 to 200 µl. Multiple gel pieces can be processed in a single tube and the final protein concentration is relatively high.
Application Extracted proteins can be used for MALD-MS analysis, immunization of animals, protein-protein interaction and protein-nucleic acid interaction studies etc. Extracted nucleic acid can be used for PCR, cloning, and sequencing etc.
Kit components

1. 20 filter cartridges with 2.0 ml collection tubes

2. 20 microfuge tubes (200 µl)

3. 2 gram extraction powder

 4. Micro pestle X 4
Storage: Store the kit at room temperature

Additional Materials Required: A Table-Top Microcentrifuge