Product: Anti-Bad (Bcl-2-like 8), polyclonal
Cat. No.: PC-466 (100 μg)
Description:
Members in the Bcl-2 family are critical
regulators of apoptosis by either inhibiting or
promoting cell death. Bcl-2 homology 3 (BH3)
domain containing pro-apoptotic proteins, such
as Bax, Bid, and Bik, form a growing subclass of
the Bcl-2 family. Another such protein is the Bcl-
2-antagonist of cell death (Bad). Bad regulates
apoptosis by forming heterodimers with antiapoptotic
proteins Bcl-2 and Bcl-xL, thereby
preventing them from binding with Bax. Bad
activity is regulated by its phosphorylation; it is
inactivated by kinases such as Akt and MAP
kinase and thus promotes cell survival, whereas
JNK-induced phosphorylation promotes the
apoptotic role of Bad.
Specificity:
Recognizes Bad.
Species Reactivity:
Human rat and mouse. Other species not tested.
Host:
Rabbit
Immunogen:
Anti-Bad polyclonal antibody was raised against
a peptide corresponding to 15 amino acids near
the C-terminus of human Bad (GenBank
accession no. Q92934).
Format:
100 μg at 1 mg/mL of ion exchange
chromatography purified IgG in PBS containing
0.02% sodium azide.
Storage:
Store at 4°C.
Applications and Suggested Dilutions:

Immunohistochemistry: Use at 2 μg/mL.

Western blot: Recommend 0.5–1.0 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1. Western blot analysis of Bad in T24 cell lysates
with anti-Bad at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Figure 2. Immunohistochemical staining of rat thymus using
anti-Bad at 2 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.