核连蛋白1(NUCB1)重组蛋白

YB179Ra012
Recombinant Nucleobindin 1 (NUCB1)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
核连蛋白1(NUCB1)重组蛋白[ PROPERTIES ]
Residues: Val26~Asp413
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q02819
Host: E. coli
Subcellular Location: Golgi Apparatus. Cis-golgi
Network Membrane; Peripheral Membrane Protein;
Lumenal Side.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
核连蛋白1(NUCB1)重组蛋白determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.1
Predicted Molecular Mass: 49.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
核连蛋白1(NUCB1)重组蛋白Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
VPVDR AAPPQEDSQA TETPDTGLYY HRYLQEVINV LETDGHFREK LQAANAEDIK
SGKLSQELDF VSHNVRTKLD ELKRQEVSRL RMLLKAKMDA KQEPNLQVDH MNLLKQFEHL
DPQNQHTFEA RDLELLIQTA TRDLAQYDAA HHEEFKRYEM LKEHERRRYL ESLGEEQRKE
AERKLQEQQR RHREHPKVNV PGSQAQLKEV WEELDGLDPN RFNPKTFFIL HDINSDGVLD
EQELEALFTK ELEKVYDPKN EEDDMREMEE ERLRMREHVM KNVDTNQDRL VTLEEFLAST
QRKEFGDTGE GWKTVEMSPAYTEEELKRFE EELAAREAEL NARAQRLSQE TEALGRSQDR
LEAQKRELQQ AVLQMEQRKQ QLQEQSAPPS KPD