嘌呤霉素（PURO-1）

Description

Puromycin is an aminonucleoside antibiotic isolated from Streptomyces alboniger. Puromycin terminates protein synthesis at the level of translation in prokaryotic and eukaryotic cells and is active against gram-positive bacteria and weakly active against gram-negative bacteria. Resistance to puromycin is conferred by the puromycin N-acetyl-transferase (pac) gene [1, 2]. Exogenous expression of the pac gene is commonly used in conjunction with puromycin to generate stable cell lines.

Most working concentrations range from 1-10 μg/ml; however, the optimal concentration should be determined for individual cell types and culture conditions. In most cases, puromycin kills 99% of non-resistant cells within 48 hours.

Product Use
PURO-1 is for research use only. It is not approved for human or animal use, or for application in clinical or in vitro diagnostic procedures.

Storage
Store at -20°C; avoid freeze-thaw cycles.

Shipping
Dry ice.

Reference
[1] Vara J, Perez-Gonzalez JA, Jimenez A. (1985) “Biosynthesis of puromycin by Streptomyces alboniger: characterization of puromycin N-acetyltransferase”. Biochemistry 24: 8074-8081.
[2] Lacalle RA, Pulido D, Vara J, Zalacain M, Jimenez A. (1989) “Molecular analysis of the pac gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger”. Gene 79: 375-380.