大鼠支链α-酮酸脱氢酶(BCKDK)多克隆抗体

Polyclonal Antibody to Branched Chain Alpha-Ketoacid Dehydrogenase Kinase (BCKDK)

[ PROPERTIES ]

Source: Polyclonal antibody preparation

Host: Rabbit

Purification: Antigen-specific Affinity Chromatography. Traits: Liquid

Concentration: 200µg/mL

UOM: 100µg

Applications: WB; IHC; ICC; IP.

[ IMMUNOGEN ]

Immunogen: Recombinant BCKDK (Arg44~Thr223) expressed in E.coli

[ APPLICATIONS ]

Western blotting: 0.5-2µg/mL

Immunohistochemistry: 5-20µg/mL

Immunocytochemistry: 5-20µg/mL

Optimal working dilutions must be determined by end user

[ FORMULATION ]

Form & Buffer: Supplied as solution form in 0.01M PBS, pH7.4, containing0.05% Proclin-300, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant BCKDK disposed in loading

buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 4ºC for frequent use.

Aliquot and store at -20ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ IDENTIFICATION ]

本产品仅供科研使用.请勿用于医药,不能用于临床治疗诊断使用！以实际收货产品说明书为准，网站说明书仅供参考。