ATP4B,氢钾ATP酶通道蛋白抗体

产品编号HZ-2433R
英文名称ATP4B
中文名称氢钾ATP酶通道蛋白抗体
别 名Gastric H(+)/K(+) ATPase subunit beta; H+K+ ATPase; ATP4B; ATP6B; ATPase H+/K+ exchanging beta polypeptide; ATPase H+/K+ transporting beta polypeptide;Gastric H K ATPase catalytic subunit; Gastric H+/K+ ATPase beta subunit; Gastric hydrogen potassium ATPase; Gastric hydrogen potassium ATPase beta; Hydrogen/potassium exchanging ATPase 4B; Potassium transporting ATPase beta chain; Potassium transporting ATPase subunit beta; Proton pump; Proton pump beta chain; ATP4B_HUMAN.
规格价格0.1ml 0.2ml 
说 明 书0.1ml 0.2ml
研究领域细胞生物 免疫学 转录调节因子
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
ATP4B,氢钾ATP酶通道蛋白抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量33kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human ATP4B
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
ATP4B,氢钾ATP酶通道蛋白抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008]

Function:
Required for stabilization and maturation of the catalytic proton pump alpha subunit and may also involved in cell adhesion and establishing epithelial cell polarity.

Subunit:
Composed of two subunits: alpha (catalytic) and beta.

Subcellular Location:
Cell membrane

Tissue Specificity:
Expressed at highest levels in heart, skeletal

ATP4B,氢钾ATP酶通道蛋白抗体Similarity:
Belongs to the X(+)/potassium ATPases subunit beta family.

Gene ID:
496

Database links:
Entrez Gene: 496 Human
Entrez Gene: 11945 Mouse
Entrez Gene: 397131 Pig
Entrez Gene: 24217 Rat
Omim: 137217 Human
SwissProt: P51164 Human
SwissProt: P50992 Mouse
SwissProt: P18434 Pig
SwissProt: P18597 Rabbit
SwissProt: P18598 Rat
Unigene: 434202 Human
Unigene: 154039 Mouse
Unigene: 10033 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 

产品图片

Tissue/cell: mouse heart tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-ATP4B Polyclonal Antibody, Unconjugated(bs-2433R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining