MAGI1,膜相关鸟苷酸反转激酶1抗体

产品编号HZ-11352R
英文名称MAGI1
中文名称膜相关鸟苷酸反转激酶1抗体
别 名MAGI1_HUMAN; Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1; Atrophin-1-interacting 3; AIP-3; BAI1-associated protein 1; BAP-1; Membrane-associated guanylate kinase inverted 1; MAGI-1; Trinucleotide repeat-containing gene 19 protein; WW domain-containing protein 3; WWP3; MAGI1; AIP3; BAIAP1; BAP1; TNRC19.
说 明 书0.1ml 0.2ml
研究领域细胞生物 神经生物学 信号转导 激酶和磷酸酶 细胞骨架 细胞外基质
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit, Sheep,
MAGI1,膜相关鸟苷酸反转激酶1抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量137kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human MAGI1
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
MAGI1,膜相关鸟苷酸反转激酶1抗体PubMedPubMed
产品介绍background:
The membrane-associated guanylate kinase (MAGUK) proteins are concentrated at the membrane-cytoskeletal interface where they facilitate the assembly of multiprotein complexes on the inner surface of the plasma membrane. Three protein-protein interaction modules characteristically define MAGUK related proteins: the PDZ domain, the SH3 domain and the guanylate kinase (GuK) domain. The closely related MAGUK proteins, MAGI-1, MAGI-2 and MAGI-3 (membrane associated guanylate kinase inverted-1 and 2), likewise contain the GuK domain and five PDZ domains; however, the SH3 domain is replaced with a WW domain. The transcripts of MAGI-1 are alternatively spliced to produce three distinct proteins having unique C-terminals. Two variants, MAGI-1a and MAGI-1b, are associated with the membrane and cytosolic fractions and are primarily expressed in the brain. The third isoform, MAGI-1c, encodes for a nuclear localization signal that localizes MAGI-1c to the nucleus, and it is primarily expressed in the liver and kidney. MAGI-2 and MAGI-3 are localized to the plasma membrane, and they contribute to protein scaffolding by associating with the protein phosphatase PTEN.

Function:
May play a role as scaffolding protein at cell-cell junctions. May regulate acid-induced ASIC3 currents by modulating its expression at the cell surface (By similarity).

MAGI1,膜相关鸟苷酸反转激酶1抗体Subunit:
Interacts through its WW 2 domain with SYNPO and through its PDZ 5 domain with ACTN4. Interacts with cytoplasmic domain of BAI1. Interacts via its WW domains with DRPLA. Interacts with ESAM, LRP2 and CXADR. May interact with CTNNB1. Interacts through its PDZ 1 domain with NET1 (By similarity). Interacts with ASIC3 and AMOT. Interacts with FCHSD2. Interacts with IGSF5/JAM4 and through its PDZ 2 and 3 domains with NPHS1 forming a tripartite complex (By similarity). Interacts with DDN.

Subcellular Location:
Cell junction; cell membrane; tight junction; peripheral membrane protein. Localizes to epithelial cells tight junctions.

Tissue Specificity:
Widely expressed with the exception of skeletal muscle. Isoform 1, isoform 2 and isoform 6 are highly expressed in colon, kidney, lung, liver, and pancreas. Isoform 5 is predominantly expressed in brain and heart. Isoform 3 and isoform 4 are highly expressed in pancreas and brain.

Similarity:
Contains 1 guanylate kinase-like domain.
Contains 6 PDZ (DHR) domains.
Contains 2 WW domains.

Database links:
Entrez Gene: 9223 Human
Entrez Gene: 14924 Mouse
Omim: 602625 Human
SwissProt: Q96QZ7 Human
SwissProt: Q6RHR9 Mouse

MAGI1,膜相关鸟苷酸反转激酶1抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.