KLHL13,kelch样蛋白13抗体

产品编号HZ-7752R
英文名称KLHL13
中文名称kelch样蛋白13抗体
别 名Kelch-like protein 13; BTB and kelch domain-containing protein 2; KLH13_HUMAN.
说 明 书0.1ml 0.2ml
研究领域细胞生物 细胞周期蛋白 细胞分化 表观遗传学
抗体来源Rabbit
克隆类型Polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, Zebrafish
KLHL13,kelch样蛋白13抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量74kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human KLHL13
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
KLHL13,kelch样蛋白13抗体PubMedPubMed
产品介绍background:
KLHL13 (kelch like 13), also known as BKLHD2 (BTB and kelch domain containing protein 2), is a 604 amino acid protein that contains six Kelch repeats and one BTB/POZ domain. Expressed predominantly in brain, KLHL13 is believed to play a role in protein ubiquitination and may function as a substrate specific adapter of an E3 ubiquitin protein ligase complex. E3 ligases accept a ubiquitin residue from an E2 ubiquitin conjugating enzyme and immediately transfer that residue to a protein that is targeted for degradation. Specifically, KLHL13 interacts with KLHL9 and CUL3, a member of the cullin family of mediators that participate in the selective targeting of proteins for ubiquitin mediated proteolysis. Via its BTB and C terminal Kelch (BACK) motif, KLHL13 is thought to play a role in spatially orientating substrates in the CUL3 ligase.

Function:
Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for mitotic progression and cytokinesis. The BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex mediates the ubiquitination of AURKB and controls the dynamic behavior of AURKB on mitotic chromosomes and thereby coordinates faithful mitotic progression and completion of cytokinesis.

Subunit:
Component of the BCR(KLHL9-KLHL13) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL9, KLHL13 and RBX1. Interacts with AURKB.

KLHL13,kelch样蛋白13抗体Similarity:
Contains 1 BACK (BTB/Kelch associated) domain.
Contains 1 BTB (POZ) domain.
Contains 6 Kelch repeats.

Database links:
UniProtKB/Swiss-Prot: Q9P2N7.3

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.