ATP结合盒转运蛋白B11(ABCB11)重组蛋白

YB641Mu013
Recombinant ATP Binding Cassette Transporter B11 (ABCB11)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
 ATP结合盒转运蛋白B11(ABCB11)重组蛋白9th Edition (Revised in Jul, 2013)
[ PROPERTIES ]
Residues: Ile420~Leu656 (Accession # Q9QY30),
with two N-terminal Tags, His-tag and T7-tag. Host: E. coli
Subcellular Location: Membrane; Multi-pass
membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
 ATP结合盒转运蛋白B11(ABCB11)重组蛋白Predicted isoelectric point: 6.6
Predicted Molecular Mass: 30.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
 ATP结合盒转运蛋白B11(ABCB11)重组蛋白[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its
sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-I EFHNVTFHYP SRPEVKILNN
LSMVIKPGET TAFVGSSGAG KSTALQLIQR FYDPCEGMVT LDGHDIRSLN IRWLRDQIGI
VEQEPVLFST TIAENIRLGR EEATMEDIVQ AAKDANAYNF IMALPQQFDT LVGEGGGQMS
GGQKQRVAIA RALIRKPKIL LLDMATSALD NESEAKVQGA LNKIQHGHTI ISVAHRLSTV
RSADVIIGFE HGTAVERGTH EELLERKGVY FMLVTL