phospho-Caseine Kinase 1 alpha(Tyr294),磷酸化酪蛋白激酶casein kinase Iα抗体

产品编号HZ-12428R
英文名称phospho-Caseine Kinase 1 alpha(Tyr294)
中文名称磷酸化酪蛋白激酶casein kinase Iα抗体
别 名Caseine Kinase 1 alpha (phospho Y294); p-CK1A (phospho Y294); Casein kinase 1 alpha 1; Casein kinase I isoform alpha; CK1; CK1A; CKI alpha; CKI-alpha; CKIa; Clock regulator kinase; Csnk1a1; Down regulated in lung cancer; HLCDGP1; KC1A_HUMAN; PRO2975.
说 明 书0.1ml
产品类型磷酸化抗体
研究领域肿瘤 细胞生物 信号转导 激酶和磷酸酶
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Rabbit, Sheep,
phospho-Caseine Kinase 1 alpha(Tyr294),磷酸化酪蛋白激酶casein kinase Iα抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量39kDa
细胞定位细胞核 细胞浆
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthesised phosphopeptide derived from human Caseine Kinase 1 alpha around the phosphorylation site of Tyr294
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-Caseine Kinase 1 alpha(Tyr294),磷酸化酪蛋白激酶casein kinase Iα抗体PubMedPubMed
产品介绍background:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

Function:
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.

Subunit:
Monomer. Interacts with the Axin complex. Interacts with TUT1, leading to TUT1 phosphorylation.

Subcellular Location:
Cytoplasm. Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Nucleus speckle. Note=Localizes to the centrosome in interphase cells, and to kinetochore fibers during mitosis.

Similarity:
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family.
Casein kinase I subfamily.
Contains 1 protein kinase domain.

phospho-Caseine Kinase 1 alpha(Tyr294),磷酸化酪蛋白激酶casein kinase Iα抗体Database links:
UniProtKB/Swiss-Prot: P48729.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-phospho-Caseine Kinase 1 alpha (Tyr294) Polyclonal Antibody, Unconjugated(HZ-12428R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining