胎牛血清(胚胎干细胞专用)
产品名称: 胎牛血清(胚胎干细胞专用)
英文名称: Fetal Bovine Serum (FBS), US Origin, Qualified for Human Embryonic Stem Cells
产品编号: OM296337
产品价格: 0
产品产地: 详询
品牌商标: Omnimabs
更新时间: null
使用范围: null
北京百奥创新科技有限公司
- 联系人 :
- 地址 : 北京市海淀区创客小镇
- 邮编 :
- 所在区域 : 北京
- 电话 : 133****0421 点击查看
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- 邮箱 : customer@bestopbio.com;3007606172@qq.com
Product Name: Fetal Bovine Serum (FBS), US Origin, Qualified for Human Embryonic Stem Cells. Product description :Project OverviewEmbryonic Stem (ES) Cells are pluripotent cells derived from the inner cell mass of the blastocyst. The stem cells can be maintained in vitro for extended periods without loss of their capacity to differentiate to all cell lineages when reimplanted back into a blastocyst. ES cells may differentiate in vitro to a variety of cell types including neuronal, muscle, endothelial and hematopoietic progenitors. General culture conditions are well established and usually require ES cells to be grown on an inactive feeder cell layer or with basement membrane components (Matrigel, Fibronectin, Laminin). When growing ES cells, one of the most important parameters is the maintenance of the cells in the undifferentiated state. Pre-screening of the serum is essential before using it for the culture of ES cells. Various lots of sera are screened for the growth of Human ES cells using MEF's as a feeder layer. The following parameters are measured for the screening:Human ES cells colony morphologyPlating efficiencyDifferentiation rate: analysis of Human ES cells surface marker expressed on the undifferentiated cells membrane (FACS analysis). The results are used as an indication of the quality of sera for the growth and maintenance of undifferentiated stem cells. Isotype :Cocktail. Host Species :Alligator. Purification :Affinity purified.