phospho-ATP citrate lyase,磷酸化三磷酸腺柠檬酸裂解酶抗体

产品编号HZ-3037R
英文名称phospho-ATP citrate lyase (Ser455)
中文名称磷酸化三磷酸腺柠檬酸裂解酶抗体
别 名ATP citrate lyase (phospho S455); ACL; ATP citrate (pro-S) lyase; ATP citrate synthase; Citrate cleavage enzyme; EC 2.3.3.8; A730098H14RIK; ACLY; ATP CITRATE LYASE; ATPCL; AW538652; Cce; CITRATE LYASE; CLATP; MGC124629; ACL; Acly; ACLY_HUMAN; ATP citrate lyase; ATP-citrate (pro-S-)-lyase; ATP-citrate synthase; ATPcitrate synthase; OTTHUMP00000164773.
规格价格0.1ml
说 明 书0.1ml
产品类型磷酸化抗体
研究领域肿瘤 激酶和磷酸酶
phospho-ATP citrate lyase,磷酸化三磷酸腺柠檬酸裂解酶抗体抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Sheep,
产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-200 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量122kDa
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated Synthesised phosphopeptide derived from human ATP citrate lyase around the phosphorylation site of Ser455
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
phospho-ATP citrate lyase,磷酸化三磷酸腺柠檬酸裂解酶抗体PubMedPubMed
产品介绍background:
ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Function:
ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

Subunit:
Homotetramer.

Subcellular Location:
Cytoplasm.

phospho-ATP citrate lyase,磷酸化三磷酸腺柠檬酸裂解酶抗体Post-translational modifications:
ISGylated.
Acetylated at Lys-540, Lys-546 and Lys-554 by KAT2B/PCAF. Acetylation is promoted by glucose and stabilizes the protein, probably by preventing ubiquitination at the same sites. Acetylation promotes de novo lipid synthesis. Deacetylated by SIRT2.
Ubiquitinated at Lys-540, Lys-546 and Lys-554 by UBR4, leading to its degradation. Ubiquitination is probably inhibited by acetylation at same site.

Similarity:
In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.
In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.

Gene ID:
47

Database links:
Entrez Gene: 47 Human
Entrez Gene: 104112 Mouse
Entrez Gene: 24159 Rat
Omim: 108728 Human
SwissProt: P53396 Human
SwissProt: Q91V92 Mouse
SwissProt: P16638 Rat
Unigene: 387567 Human
Unigene: 282039 Mouse
Unigene: 29771 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.