188kDa核孔蛋白(NUP188)重组蛋白

YB183Hu012
Nucleoporin Nucleoporin Nucleoporin Nucleoporin 188kDa (NUP188) (NUP188) (NUP188) (NUP188)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
5th Edition (Revised in January, 2013)
188kDa核孔蛋白(NUP188)重组蛋白[ DESCRIPTION DESCRIPTION DESCRIPTION DESCRIPTION ]
Protein Protein Protein Protein Names: Nucleoporin 188kDa
Synonyms: Synonyms: Synonyms: Synonyms: NUP188, KIAA0169
Species: Species: Species: Species: Human
Size: 100µg
Source: Source: Source: Source: Escherichia coli-derived
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Nucleus, nuclear pore
complex.
188kDa核孔蛋白(NUP188)重组蛋白[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Thr540~Gly782 (Accession # Q5SRE5),
with two N-terminal Tags, His-tag and GST-tag. Grade & Purity: Purity: Purity: Purity: >95%, 55kDa as determined by
SDS-PAGE reducing conditions.
Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS, pH
7.4, containing 5% sucrose, 0.01% sarcosyl. Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0 EU per 1μg (determined by
the LAL method).
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 53.8kDa
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 6.4
Human NUP188
[ PREPARATION PREPARATION PREPARATION PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
188kDa核孔蛋白(NUP188)重组蛋白Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL
DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD
VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD
GS T S GS G H H H HH HSAG LVP R GST AIG MKET AAAK F E R Q HM DSP D LG T LEV
LFQGPLGSEF- T LFTCEIEMLL HVVSTADVIQ HCQRVKPIID LVHKVISTDL SIADCLLPIT
SRIYMLLQRL TTVISPPVDV IASCVNCLTV LAARNPAKVW TDLRHTGFLP FVAHPVSSLS
QMISAEGMNA GGYGNLLMNS EQPQGEYGVT IAFLRLITTL VKGQLGSTQS QGLVPCVMFV
LKEMLPSYHK WRYNSHGVRE QIGCLILELI HAILNLCHET DLHSSHTPSL QFLCICSLAY
TEAGQTVINI MG