Application
Coating buffer: Use coating buffer to dilute the sample you will coat, add 100μL/well to high-bind plate.

Washing buffer: Dilute the washing buffer to 1x with distilled water before experiment, add 300μL/well for washing the coated plate.

Blocking buffer: Dilute the washing buffer to 1x with distilled water before experiment, add 300μL/well for blocking the coated plate. The samples and detection antibodies can be prepared by 1x blocking buffer which should be 4 times dilution.

Substrate solution: Add 100μL/well to plate.

Stop solution: Add 50μL/well to plate. 
Storage
Store this set at 2-8℃, and protect from light. If precipitation occurs, filter to clarify before use.