肝细胞核因子4α(HNF4α)重组蛋白

YB418Hu015
Recombinant Hepatocyte Nuclear Factor 4 Alpha (HNF4a)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
 肝细胞核因子4α(HNF4α)重组蛋白[ PROPERTIES ]
Residues: Gln131~Ser330
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: P41235
Host: E. coli
Subcellular Location: Nucleus.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,
 肝细胞核因子4α(HNF4α)重组蛋白150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,
0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 26.5kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
 肝细胞核因子4α(HNF4α)重组蛋白[ SEQUENCES ]
The sequence of the target protein is listed below.
QNERDRISTR RSSYEDSSLP SINALLQAEV LSRQITSPVS GINGDIRAKK IASIADVCES
MKEQLLVLVE WAKYIPAFCE LPLDDQVALL RAHAGEHLLL GATKRSMVFK DVLLLGNDYI
VPRHCPELAE MSRVSIRILD ELVLPFQELQ IDDNEYAYLK AIIFFDPDAK GLSDPGKIKR
LRSQVQVSLE DYINDRQYDS
[ REFERENCES ]
1. Kritis A.A., et al. (1996) Gene 173:275-280. 2. Drewes T., et al. (1996) Mol. Cell. Biol. 16:925-931. 3. Yamagata K., et al. (1996) Nature 384:458-460. 4. Chartier F.L., et al. (1994) Gene 147:269-272.