肝表达抗菌肽2(LEAP2)重组蛋白

YB750Hu015
Liver Expressed Expressed Expressed Expressed Antimicrobial Antimicrobial Antimicrobial Antimicrobial Peptide Peptide Peptide Peptide 2(LEAP2) 2(LEAP2) 2(LEAP2) 2(LEAP2)
Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)
Instruction Instruction Instruction Instruction manual
肝表达抗菌肽2(LEAP2)重组蛋白NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
7th Edition (Revised in May, 2013)
[ PROPERTIES PROPERTIES PROPERTIES PROPERTIES ]
Residues: Residues: Residues: Residues: Ser23~Gln76 (Accession # Q969E1),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Subcellular Subcellular Subcellular Location: Location: Location: Location: Secreted.
Purity: Purity: Purity: Purity: >95%
Endotoxin Endotoxin Endotoxin Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
肝表达抗菌肽2(LEAP2)重组蛋白Formulation: Formulation: Formulation: Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Predicted Predicted Predicted Predicted isoelectric isoelectric isoelectric isoelectric point: 8.8
Predicted Predicted Predicted Predicted Molecular Molecular Molecular Molecular Mass: 33.2kDa
Accurate Accurate Accurate Accurate Molecular Molecular Molecular Molecular Mass: 39kDa as determined by SDS-PAGE reducing conditions.
Applications: Applications: Applications: Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
肝表达抗菌肽2(LEAP2)重组蛋白3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to
give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE STORAGE STORAGE STORAGE AND STABILITY STABILITY STABILITY STABILITY ]
Storage: Storage: Storage: Storage: Avoid repeated repeated repeated repeated freeze/thaw freeze/thaw freeze/thaw freeze/thaw cycles. cycles. cycles. cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Stability Stability Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES SEQUENCES SEQUENCES SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL
DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD
VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD
GS T S G S G H H H HH HSA G LVP R GS TA IG MK E T AA AK F E R Q HM DSP D L G T L EV
LFQGPLGSEF-SPIPEVSS AKRRPRRMTP FWRGVSLRPI GASCRDDSEC ITRLCRKRRC
SLSVAQ
[ REFERENCES REFERENCES REFERENCES REFERENCES ]
1. Rose J.E., et al. (2010) Mol. Med. 16:247-253. 2. Howard A., et al. (2010) Cell. Immunol. 261:128-133. 3. Hocquellet A., et al. (2010) Peptides 31:58-66. 4. Henriques S.T., et al. (2010) ChemBioChem 11:2148-2157.