分子量:73.2 kDa

纯度:>97% as determined by reduced SDS-PAGE. 

内毒素:Less than 1.0 EU per μg of the Rat PCSK9, His Tag by the LAL method.

Buffer:PBS,pH7.4

生物活性:Measured by its binding ability in a functional ELISA.
Immobilized Human LDL R, Strep Tag (Cat# LDR-H5281) at 10 μg/mL (100 μl/well) can bind Rat PCSK9, His Tag (Cat# PC9-R52H4 ) with a linear range of 2-15 ng/mL.

产品特性:Rat PCSK9, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 73.2 kDa. The predicted N-terminus is Gln 31. The reducing (R) protein migrates as 22 and 66 kDa in SDS-PAGE  due to glycosylation.

产品背景:Proprotein convertase subtilisin/kexin type 9 (PCSK9), is an enzyme which in humans is encoded by the PCSK9 gene.This gene encodes a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein plays a major regulatory role in cholesterol homeostasis. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation. PCSK9 may also have a role in the differentiation of cortical neurons. Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).