血幼素(HJV)重组蛋白

YB995Ra011
Hemojuvelin (HJV)
Organism: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES
4th Edition (Revised in February, 2012)
 血幼素(HJV)重组蛋白[ DESCRIPTION ]
Protein Names: Hemojuvelin
Synonyms: HFE2, HJV, RGMC
Species: Human
Size: 100µg
Source: Escherichia coli-derived
Subcellular Location: Secreted.
 血幼素(HJV)重组蛋白[ PROPERTIES ]
Residues: Lys234~Ser416 (Accession # Q6ZVN8), with N-terminal His-Tag.
Grade & Purity: >95%, 22 kDa as determined by SDS-PAGE reducing conditions.
Formulation: Supplied as lyophilized form in PBS, pH 7.4, containing 0.01% Sarcosyl,
5% sucrose.
Endotoxin Level: <1.0 EU per 1µg (determined by the LAL method).
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted Molecular Mass: 20.96 kDa
Predicted isoelectric point: 5.36
Human HJV kDa
70
44
33
27
18
14
10
15% SDS-PAGE
[ PREPARATION ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
 血幼素(HJV)重组蛋白[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months .
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was
determined by accelerated thermal degradation test, that is, incubate the protein at 37oC or 48h, and no obvious
degradation and precipitation were observed. (Referring from China Biological Products Standard, which was
calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCES]
The target protein is fused with N-terminal His-tag, its sequence is listed below.
MGHHHHHHSGSEF-KVYQAEVDNLPVAFEDGSINGGDRPGGSSLSIQTANPGNHVEIQAAYIGTTIIIRQTAGQLS
FSIKVAEDVAMAFSAEQDLQLCVGGCPPSQRLSRSERNRRGAITIDTARRLCKEGLPVEDAYFHSCVFDVLISGD
PNFTVAAQAALEDARAFLPDLEKLHLFPSDAGVPLSSATLLAPLLS
[ REFERENCES ]
1. Papanikolaou G.,et al. (2004) Nat. Genet. 36:77-82.
2. Ota T., et al. (2004) Nat. Genet. 36:40-45.
3. Gregory S.G., et al. (2006) Nature. 441:315-321.
4. Lanzara C., et al. (2004) Blood. 103:4317-4321.