ASCC2 抗体
Applications
| In-house applications |
ELISA, IHC, IF, WB, FC, IP Application not tested? Please see our Guarantee. |
| Species specificity |
Human,Mouse,Rat; other species not tested. |
|
Images
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 Immunohistochemical of paraffin-embedded human colon cancer using 11529-1-AP(ASCC2 antibody) at dilution of 1:100 (under 10x lens) Immunofluorescent analysis of HepG2 cells, using ASCC2 antibody 11529-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). HeLa cells were subjected to SDS PAGE followed by western blot with 11529-1-AP( ASCC2 Antibody) at dilution of 1:1000 IP Result of anti-ASCC2 (IP:11529-1-AP, 4ug; Detection:11529-1-AP 1:1000) with HeLa cells lysate 2640ug. 1X10^6 HepG2 cells were stained with 0.2ug ASCC2 antibody (11529-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
|
| Positive WB detected in |
HeLa cells, human brain tissue, human colon tissue, mouse colon tissue |
| Positive IP detected in |
HeLa cells |
| Positive IHC detected in |
Human colon cancer tissue |
| Positive IF detected in |
HepG2 cells |
| Positive FC detected in |
HepG2 cells |
| Recommended dilution |
WB : 1:500-1:5000
IP : 1:500-1:5000
IHC : 1:20-1:200
IF : 1:10-1:100 |
ASCC2 抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
| Source |
Rabbit |
Purification method |
Antigen affinity purification |
| Isotype |
IgG |
Storage |
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information ASCC2 抗体
| Immunogen |
ASCC2 fusion protein ag2100 |
Full name |
Activating signal cointegrator 1 complex subunit 2 |
Calculated molecular
weight |
757aa,86 kDa |
Observed molecular
weight |
90-100 kDa |
GenBank accession
number |
BC025368 |
Gene ID (NCBI) |
84164 |
| Gene symbol |
ASCC2 |
Synonyms |
ASC 1 complex subunit p100, ASC1p100, ASCC2, Trip4 complex subunit p100 ASCC2 抗体 |
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