• 特异性aAb181249 recognizes 2 isoforms of PEG10.
• 经测试应用WB, ICC/IFmore details
• 种属反应性
  与反应: Mouse, Rat, Human
• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PEG10 aa 100-200. The exact sequence is proprietary.
  Database link: Q86TG7

• 阳性对照
  • JAR, 293, HeLa and HepG2 cell lysates; HepG2 cells.
• 常规说明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid
• 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
• 存储溶液Preservative: 0.01% Sodium azide
  Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
• 浓度100 µl 浓度为 0.721 mg/ml 40 µl 浓度为 0.721 mg/ml 10 µl 浓度为 0.721 mg/ml 
  
• 纯度Protein A purified
• 克隆单克隆
• 克隆编号EPR8156(2)
• 同种型IgG
• 研究领域
  • Epigenetics and Nuclear Signaling
   
  • Transcription
   
  • Domain Families
  • Zinc Finger

  • Epigenetics and Nuclear Signaling
   
  • Transcription
   
  • Transcription Factors

  • Cancer
   
  • Oncoproteins/suppressors
   
  • Viral proteins
   
  • Retrovirus

  • Stem Cells
   
  • Germline Stem Cells
   
  • Spermatogonial Stem Cells

应用

靶标

• 功能Prevents apoptosis in hepatocellular carcinoma (HCC) cells through interaction with SIAH1, a mediator of apoptosis. May also have a role in cell growth promotion and hepatoma formation. Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1. When overexpressed, induces the formation of cellular extension, such as filipodia in association with ALK1. Involved at the immediate early stage of adipocyte differentiation (By similarity). May bind to the 5'-GCCTGTCTTT-3' DNA sequence of the MB1 domain in the myelin basic protein (MBP) promoter.
• 组织特异性Expressed in the cytotrophoblast layer but not in the overlying syncytiotrophoblast of the placenta. Expressed in prostate and breast carcinomas but not in normal breast and prostate epithelial cells. Expressed in the HepG2 cell line (at protein level). Expressed in brain, liver, spleen, kidney, thymus, lung, ovary, testis, reactive lymph node, skeletal muscle, adipose tissue and placenta. Expressed in pancreatic and hepatocellular carcinomas (HCC).
• 序列相似性Contains 1 CCHC-type zinc finger.
• 发展阶段Expressed in placenta during the first trimester of gestation (at protein level). In placenta, down-regulated at early hypoxic phase, and highly activated at 11-12 week of gestation.
• 翻译后修饰Isoform RF1/RF2 undergoes proteolytic cleavage.
• 细胞定位Nucleus. Cytoplasm. Detected predominantly in the cytoplasm of breast and prostate carcinomas, in hepatocellular carcinoma (HCC) and B-cell chronic lymphocytic leukemia (B-CLL) cells and in the HepG2 cell line. Colocalized with ALK1.

• Anti-PEG10 antibody [EPR8156(2)] 图像

  • 

    Immunocytochemistry/ Immunofluorescence - Anti-PEG10 [EPR8156(2)] antibody (ab181249)

    Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PEG10 with ab181249 at 1/250 dilution (red) followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • 

    Western blot - Anti-PEG10 [EPR8156(2)] antibody (ab181249)
    All lanes : Anti-PEG10 antibody [EPR8156(2)] (ab181249) at 1/2000 dilution
    
    Lane 1 : JAR cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HepG2 cell lysate
    
    Lysates/proteins at 20 µg per lane.
    
    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
    
    Predicted band size : 80 kDa