Cyclin B3,周期素B3抗体

产品编号HZ-7884R
英文名称Cyclin B3
中文名称周期素B3抗体
别 名CCNB 3; CCNB3; CYCB3; G2/mitotic specific cyclin B3.
说 明 书0.1ml 0.2ml
研究领域细胞生物 染色质和核信号 细胞周期蛋白
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse, Sheep,
Cyclin B3,周期素B3抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量158kDa
细胞定位细胞核
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Cyclin B3
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Cyclin B3,周期素B3抗体PubMedPubMed
产品介绍background:
The protein encoded by this gene belongs to the highlyconserved cyclin family, whose members are characterized by adramatic periodicity in protein abundance through the cell cycle.Cyclins function as positive regulators of cyclin-dependent kinases(CDKs), and thereby play an essential role in the control of thecell cycle. Different cyclins exhibit distinct expression anddegradation patterns, which contribute to the temporal coordinationof each mitotic event. Studies of similar genes in chicken anddrosophila suggest that this cyclin may associate with CDC2 andCDK2 kinases, and may be required for proper spindle reorganizationand restoration of the interphase nucleus. Alternatively splicedtranscript variants encoding different isoforms have been describedfor this gene.

Function:
Cyclins are positive regulatory subunits of thecyclin-dependent kinases (CDKs), and thereby play an essential rolein the control of the cell cycle, notably via their destructionduring cell division. Its tissue specificity suggest that it may berequired during early meiotic prophase I.

Subunit:
Interacts with CDK2 kinase.

Subcellular Location:
Nucleus.

Tissue Specificity:
Testis specific. In testis, it is expressed indeveloping germ cells, but not in Leydig cells. Weakly or notexpressed in other tissues.

Post-translational modifications:
Ubiquitinated (Probable). Ubiquitination leads to itsdegradation during anaphase entry, after degradation of CCNB1.

Cyclin B3,周期素B3抗体Similarity:
Belongs to the cyclin family. Cyclin AB subfamily.

Database links:
UniProtKB/Swiss-Prot: Q8WWL7.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-Cyclin B3 Polyclonal Antibody, Unconjugated(HZ-7884R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining