DLC1 + DLC2,肝癌缺失基因1/2抗体

产品编号HZ-14340R
英文名称DLC1 + DLC2
中文名称肝癌缺失基因1/2抗体
别 名ARHGAP 7; ARHGAP7; Deleted in liver cancer 1; Deleted in liver cancer 1 protein; DLC 1; Dlc-1; DLC1; FLJ21120; HP; HP protein; KIAA1723; p122 RhoGAP; RHG07_HUMAN; Rho GTPase activating protein 7; Rho GTPase-activating protein 7; Rho type GTPase activating protein 7; Rho-type GTPase-activating protein 7; StAR related lipid transfer protein 12; StAR-related lipid transfer protein 12; STARD 12; StARD12; START domain containing protein 12; START domain-containing protein 12; STA13_HUMAN; StAR-related lipid transfer protein 13; 46H23.2; Deleted in liver cancer 2 protein; DLC-2; AltName: Full=Rho GTPase-activating protein; START domain-containing protein 13; StARD13.
说 明 书0.1ml 0.2ml
研究领域肿瘤 细胞生物 信号转导 线粒体 表观遗传学 G蛋白信号
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat, Rabbit, Sheep,
DLC1 + DLC2,肝癌缺失基因1/2抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量171/122kDa
细胞定位细胞浆 细胞膜 线粒体
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human Rho GTPase-activating protein 7
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
DLC1 + DLC2,肝癌缺失基因1/2抗体PubMedPubMed
产品介绍background:
This gene encodes a GTPase-activating protein (GAP) that is a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins. GAP family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. This gene functions as a tumor suppressor gene in a number of common cancers, including prostate, lung, colorectal, and breast cancers. Multiple transcript variants due to alternative promoters and alternative splicing have been found for this gene.[provided by RefSeq, Apr 2010]

Function:
Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality.

Subunit:
Interacts with EF1A1, facilitates EF1A1 distribution to the membrane periphery and ruffles upon growth factor stimulation and suppresses cell migration.

Subcellular Location:
Cytoplasm. Cell junction, focal adhesion. Membrane; Peripheral membrane protein. Note=Colocalizes with EF1A1 at actin-rich regions in the cell periphery.

Tissue Specificity:
Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.

DLC1 + DLC2,肝癌缺失基因1/2抗体Post-translational modifications:
Phosphorylation at Ser-88 appears to control the dimer-monomer transition. According to PubMed:15193260, it is phosphorylated at Ser-88 by PAK1, however, according to PubMed:18650427, the DYNLL1 dimer is not accessible for PAK1 and the phosphorylation could not be demonstrated in vitro.

Similarity:
Contains 1 Rho-GAP domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 START domain.

Gene ID:
10395
90627

Database links:
Entrez Gene: 10395 Human
Entrez Gene: 90627 Human
Entrez Gene: 50768 Mouse
Omim: 604258 Human
Omim: 609866 Human
SwissProt: Q96QB1 Human
SwissProt: Q9Y3M8 Human
SwissProt: Q9R0Z9 Mouse
Unigene: 134296 Human
Unigene: 210875 Mouse
SwissProt: P63170 Rat
Unigene: 5120 Human
Unigene: 256858 Mouse
Unigene: 35769 Rat
Unigene: 507704 Human
Unigene: 721224 Human

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.