Afadin,丝状肌动蛋白结合蛋白抗体

产品编号HZ-2754R
英文名称Afadin
中文名称丝状肌动蛋白结合蛋白抗体
别 名p-Afadin(Ser1718); MLLT4; Protein AF6; AF6; Afadin; AFAD_HUMAN; ALL1-fused gene from chromosome 6 protein; Protein AF-6.
规格价格0.1ml 0.2ml 
说 明 书0.1ml 0.2ml
研究领域神经生物学 信号转导 细胞粘附分子 细胞骨架
抗体来源Rabbit
克隆类型Polyclonal
Afadin,丝状肌动蛋白结合蛋白抗体交叉反应Human, Mouse, Rat, Dog, Pig, Cow, Horse,
产品应用ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量136kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human AF6 (391-430aa)
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Afadin,丝状肌动蛋白结合蛋白抗体PubMedPubMed
产品介绍background:
Afadin is a cell-cell adherens junction F-actin binding multidomain protein. Two splice variants of afadin have been described: l-afadin, which is ubiquitously expressed, and the smaller s-afadin, which is abundantly expressed in neural tissue. l-Afadin contains one PDZ domain near its middle followed by three proline-rich and one F-actin-binding region at the carboxyl-terminal. l/s-Afadin is localized to cell-cell junctions and binds several cytoplasmic proteins such as the small GTPase Ras, the tight junction protein ZO-1, and the vinculin binding protein ponsin/SH3P12.

Function:
Belongs to an adhesion system, probably together with the E-cadherin-catenin system, which plays a role in the organization of homotypic, interneuronal and heterotypic cell-cell adherens junctions (AJs). Nectin- and actin-filament-binding protein that connects nectin to the actin cytoskeleton.

Subunit:
Homodimer. Interacts with F-actin, nectin and PVRL3/nectin-3. Essential for the association of nectin and E-cadherin. Isoform 1/s-afadin does not interact with F-actin. Interacts with ZO-1 and occludin, but probably in an indirect manner. Interacts with RIT1 and RIT2 (By similarity). Interacts with NRXN1 and BCR.

Subcellular Location:
Cell junction, adherens junction. Note=Not found at cell-matrix AJs.

DISEASE:
Note=A chromosomal aberration involving MLLT4 is associated with acute leukemias. Translocation t(6;11)(q27;q23) with MLL/HRX. The result is a rogue activator protein.

Afadin,丝状肌动蛋白结合蛋白抗体Similarity:
Contains 1 dilute domain.
Contains 1 FHA domain.
Contains 1 PDZ (DHR) domain.
Contains 2 Ras-associating domains

Gene ID:
4301

Database links:
Entrez Gene: 4301 Human
Entrez Gene: 17356 Mouse
Entrez Gene: 26955 Rat
Omim: 159559 Human
SwissProt: P55196 Human
SwissProt: Q9QZQ1 Mouse
SwissProt: O35889 Rat
Unigene: 614974 Human
Unigene: 644024 Human
Unigene: 708726 Human
Unigene: 728849 Human
Unigene: 59167 Mouse
Unigene: 58 Rat

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.