BstAPI
产品名称： BstAPI
英文名称： BstAPI
产品编号： HZ600370
产品价格： 0
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： null
- 联系人 : 鲍丽雯
- 地址 : 上海市闵行区闵北路88弄1-30号第22幢AQ136室
- 邮编 : 200612
- 所在区域 : 上海
- 电话 : 139****0749 点击查看
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- 邮箱 : www.shzbio.net
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BstAPI
产品描述
使用说明
高浓度may result in星的酶活性。在Activiti 37°C is from 50%最大。number of the minimum of that resulted in单位完成消化DNA 1μg底物is in 16 hours of 0.5。
技术规格
Unit One Unit of the definition酶水解is required to the amount of DNA 1μgλ1小时60°c in at总量在50μl反应。
网站gcannnn↑ntgc recognition
嗜热脂肪芽孢杆菌来源美联社
我们assayedλDNA
优化缓冲W（10 mM Tris HCl（pH 8.5（°C）；10 mM MgCl2 100毫米；NaCl；1毫米DTT。）
60°c优化研究
10毫米的存储条件的Tris-HCl（pH 7.5）；50毫米氯化钾0.1毫米1毫米；EDTA；BSA DTT；200μg／ml甘油50 %；and Store在零下20摄氏度。
ligations after 5倍overdigestion with more than 90 %的酶的DNA碎片重新ligated and can be。
非特异性水解后没有检测到孵化nonspecific activity was withλDNA 1μg of of of 5 16 hours for at UA听说是八周60°c酶。
甲基化效应不灵敏的测试
热灭活的酶灭活°C is at by潜伏期为20分钟。
campatible Apabi ^。
使用说明
High enzyme concentration may result in star activity. At 37°C activiti is 50% from maximum. The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0.5.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl. |
| Recognition sites | GCANNNN↑NTGC |
| Source | Bacillus stearothermophilus AP |
| Assayed on | Lambda DNA |
| Optimal buffer | W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
| Optimal temperature | 60°C |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; and 50% glycerol Store at -20°C. |
| Ligations | After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 60°C. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80°C for 20 mins. |
| Campatible Ends | ApaBI^ |