ERK/MAPK (phospho T202/Y204) polyclonal antibody
产品名称： ERK/MAPK (phospho T202/Y204) polyclonal antibody
英文名称： ERK/MAPK (phospho T202/Y204) polyclonal antibody
产品编号： PAB9666
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of ERK/MAPK.
- Immunogen:
- Synthetic phosphopeptide corresponding to residues surrounding T202/Y204 of rat ERK/MAPK.
- Host:
- Rabbit
- Theoretical MW (kDa):
- 42, 44
- Reactivity:
- Bovine, Chicken, Clawed frog, Dog, Human, Mouse, Primates, Rat, Zebra fish
- Form:
- Liquid
- Purification:
- Affinity purification
- Storage Buffer:
- In 10 mM HEPES, 150 mM NaCl, pH 7.5 (50% glycerol, 10% BSA)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- Western Blot (1:1000)
The optimal working dilution should be determined by the end user.
- Publication Reference
- 1.
- Molecular recognitions in the MAP kinase cascades.
Tanoue T, Nishida E.Cell Signal. 2003 May;15(5):455-62.
- 2.
- Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases.
Johnson GL, Lapadat R.Science. 2002 Dec 6;298(5600):1911-2.
- 3.
- Molecular psychology: roles for the ERK MAP kinase cascade in memory.
Adams JP, Sweatt JD.Annu Rev Pharmacol Toxicol. 2002;42:135-63.
- Applications
- Western Blot (Cell lysate)
- Western blot of human T-47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202 and Tyr204 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Thr202/Tyr204 ERK/MAPK. The immunolabeling is completely eliminated by treatment with lambda-Ptase.