分子量:19.5 kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human PD-1, Strep Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:(1) Measured by its binding ability in a functional ELISA. 
Immobilized Human PD-1, Strep Tag (Catalog # PD1-H5284) at 2 μg/mL ( 100 μl/well ) can bind Human PD-L2, Fc Tag (Catalog # PD2-H5251) with a linear range of 0.4-3 ng/mL.
(2) Measured by its binding ability in a functional ELISA. 
Immobilized Human PD-1, Strep Tag (Catalog # PD1-H5284) at 5 μg/mL ( 100 μl/well ) can bind Human PD-L1, Fc Tag (HPLC-verified) (Catalog # PD1-H5258) with a linear range of 1-50 ng/mL.


产品特性:Human PD-1, Strep Tag is fused with twin strep tag at the C-terminus, and has a calculated MW of 19.5 kDa. The predicted N-terminus is Leu 25. The reducing (R) protein migrates as 26-45 kDa in SDS-PAGE  due to glycosylation.

产品背景:Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.