CaMK2 alpha,钙/钙调素依赖蛋白激酶2α抗体

产品编号HZ-0771R
英文名称CaMK2 alpha
中文名称钙/钙调素依赖蛋白激酶2α抗体
别 名alpha CaMKII; Calcium / Calmodulin Dependent Protein Kinase II G; Calcium calmodulin dependent protein kinase CaM kinase II alpha; Calcium calmodulin dependent protein kinase II alpha-B subunit; Calcium calmodulin dependent protein kinase II; Calcium/calmodulin dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; Calcium/calmodulin-dependent protein kinase II alpha; Calcium/calmodulin-dependent protein kinase II-alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type IIA; CaM kinase II alpha chain; CaM kinase II alpha subunit; CaM kinase II beta chain; CaM kinase II beta subunit; CaM kinase II delta chain; CaM kinase II delta subunit; CaM kinase II subunit alpha; CAM2; CaMK II alpha subunit; CaMK II beta subunit; CaMK II delta subunit; CaMK-II subunit alpha; CAMK2A; CAMK2B; CAMK2D; CAMKA; CAMKB; CaMKII; CaMK 2a; CaMK2a; KCC2A_HUMAN.
说 明 书0.1ml 0.2ml
研究领域肿瘤 心血管 细胞生物 神经生物学 信号转导 激酶和磷酸酶 新陈代谢
抗体来源Rabbit
克隆类型Polyclonal
交叉反应Human, Mouse, Rat,
CaMK2 alpha,钙/钙调素依赖蛋白激酶2α抗体产品应用WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量54kDa
细胞定位细胞膜
性 状Lyophilized or Liquid
浓 度1mg/1ml
免 疫 原KLH conjugated synthetic peptide derived from human CaMK2 alpha
亚 型IgG
纯化方法affinity purified by Protein A
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
CaMK2 alpha,钙/钙调素依赖蛋白激酶2α抗体PubMedPubMed
产品介绍background:
Ca2+/CalmodulinDependent Protein Kinase II (CaM Kinase II) is a multifunctional calcium and calmodulindependent protein kinase that mediates cellular responses to a wide variety of intercellular signals. CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+homeostasis. Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II. Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduce LTP and learning.

Function:
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.

Subunit:
CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with BAALC, MPDZ, SYN1, CAMK2N2 and SYNGAP1

Subcellular Location:
Cell junction, synapse, presynaptic cell membrane. Cell junction, synapse. Note=Postsynaptic lipid rafts.

Similarity:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.

Gene ID:
815

CaMK2 alpha,钙/钙调素依赖蛋白激酶2α抗体Database links:
Entrez Gene: 282162 Cow
Entrez Gene: 525416 Cow
Entrez Gene: 530719 Cow
Entrez Gene: 532713 Cow
Entrez Gene: 815 Human
Entrez Gene: 816 Human
Entrez Gene: 817 Human
Entrez Gene: 818 Human
Entrez Gene: 108058 Mouse
Entrez Gene: 12322 Mouse
Entrez Gene: 12323 Mouse
Entrez Gene: 12325 Mouse
Entrez Gene: 171140 Rat
Entrez Gene: 24245 Rat
Entrez Gene: 24246 Rat
Entrez Gene: 25400 Rat
Omim: 114078 Human
SwissProt: Q2HJF7 Cow
SwissProt: Q3MHJ9 Cow
SwissProt: Q13554 Human
SwissProt: Q13555 Human
SwissProt: Q13557 Human
SwissProt: Q9UQM7 Human
SwissProt: P11798 Mouse
SwissProt: P28652 Mouse
SwissProt: Q6PHZ2 Mouse
SwissProt: Q923T9 Mouse
SwissProt: P08413 Rat
SwissProt: P11275 Rat
SwissProt: P11730 Rat
SwissProt: P15791 Rat
Unigene: 725992 Human
Unigene: 131530 Mouse
Unigene: 107499 Rat

CaMK2 alpha,钙/钙调素依赖蛋白激酶2α抗体Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;  Incubation: Anti-CaMK2 alpha Polyclonal Antibody, Unconjugated(HZ-0771R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining