无翅型MMTV整合位点家族成员16(WNT16)重组蛋白

YB121Hu015
Recombinant Wingless Type MMTV Integration Site Family, Member 16 (WNT16)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
 无翅型MMTV整合位点家族成员16(WNT16)重组蛋白[ PROPERTIES ]
Residues: Asn30~Lys365 (Accession # Q9UBV4),
with two N-terminal Tags, His-tag and GST-tag. Host: E. coli
Subcellular Location: Secreted, extracellular
space, extracellular matrix.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,
pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.6
 无翅型MMTV整合位点家族成员16(WNT16)重组蛋白Predicted Molecular Mass: 70.0kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its
sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
 无翅型MMTV整合位点家族成员16(WNT16)重组蛋白KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-N WMWLGIASFG
VPEKLGCANL PLNSRQKELC KRKPYLLPSI REGARLGIQE CGSQFRHERW NCMITAAATT
APMGASPLFG YELSSGTKET AFIYAVMAAG LVHSVTRSCS AGNMTECSCD TTLQNGGSAS
EGW HWGGCSD DVQYGMWFS R KF LDFPIGNT TGKE NKVL LA MN L HNNEAGR
QAVAKLMSVD CRCHGVSGSC AVKTCWKTMS SFEKIGHLLK DKYENSIQIS DKTKRKMRRR
EKDQRKIPIH KDDLLYVNKS PNYCVEDKKL GIPGTQGREC NRTSEGADGC NLLCCGRGYN
THVVRHVERC ECKFIWCCYV RCRRCESMTD VHTCK