预优化DNA转染试剂-B16-F10-克隆与表达-试剂-生物在线
济南思科生物科技有限公司
预优化DNA转染试剂-B16-F10

预优化DNA转染试剂-B16-F10

商家询价

产品名称: 预优化DNA转染试剂-B16-F10

英文名称: GenJet™ In Vitro DNA Tranfection Reagent for B16-F10 Cells

产品编号: SL100489-B16

产品价格: 0

产品产地: 美国

品牌商标: SignaGen

更新时间: null

使用范围: null

济南思科生物科技有限公司
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  • 邮编 : 250021
  • 所在区域 : 山东
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Description

GenJet™ DNA In Vitro Tranfection Reagent for B16-F10 cell is pre-optimized for transfecting B16-F10 cells. B16-F10 is a derived murine melanoma cell line, which is well-established model for the study of experimental cancer therapies. Melanomas express different tumor-associated antigens, which are potential targets for novel designs of therapeutic cancer vaccines. Melanoma-associated antigens include tyrosinase and tyrosine-related protein (TRP)-1/2 or the MAGE and BAGE class of antigens. Many immunotherapeutic protocols have been tested using the murine B16-F10 melanoma cell line that originated in the syngeneic C57BL/6 (H-2b) mouse strain.

Refer to the following optimal transfection conditions for maximal transfection efficiency on B16-F10 cells. GenJet™ reagent, 1.0 ml, is sufficient for 300 to 600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.

Summary of Optimal Transfection Conditions:
Confluence on the day of transfection
Cell culture conditions
GenJet™ (µl) : DNA (µg) Ratio
Diluent for DNA and Transfection Reagent

Incubation Time to Form GenJet™/DNA Complex
Presence of Serum/Antibiotics during Transfection
Change Medium After Transfection
Maximal Efficiency 

Transfection Results:
Reporter Gene
Plasmid

Efficiency (GFP %)


~90%
DMEM with 4.5 g/L glucose, 10% FBS

3:1
Serum-free DMEM with 4.5 g/L glucose

15 minutes at RT
Yes
Yes, ~5 Hours After Transfection
48 hours


EGFP
pEGFP-N3 (CMV promoter) 

88%

Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet.pdf