pCLE逆病毒载体质粒图谱序列抗性-载体及构建-试剂-生物在线
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pCLE逆病毒载体质粒图谱序列抗性

pCLE逆病毒载体质粒图谱序列抗性

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产品名称： pCLE逆病毒载体质粒图谱序列抗性

英文名称： pCLE 逆病毒载体质粒图谱序列抗性说明书价格报价

产品编号： Biovector105802

产品价格： 0

产品产地： Biovector Inc. USA

品牌商标： Biovector, Addgene, ATCC, Invi

更新时间： null

使用范围： null

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  • pCLE 
    (Plasmid #17703)

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    Available to Academic and Nonprofits Only

    BACKBONE

    GROWTH IN BACTERIA

    • Bacterial Resistance(s)
      Ampicillin
    • Growth Temperature
      37°C
    • Growth Strain(s)
      DH5alpha
    • Copy number
      High Copy

    SEQUENCE INFORMATION

    • Depositor Sequences
      None.
    • Addgene Sequences

    Full plasmid sequence is available only if provided by the depositing laboratory.

    GENE/INSERT

    • Gene/Insert name
      None

    CLONING INFORMATION

    • Cloning methodRestriction Enzyme
    • 5′ cloning siteBglII (not destroyed)
    • 3′ cloning siteBglII (not destroyed)
    • 5′ sequencing primerna
      • (Common Sequencing Primers)

    RESOURCE INFORMATION

    • Terms and Licenses

    DEPOSITOR COMMENTS

    pCLE includes a modified 5 LTR (CMV/MLV) to obtain higher titers, as well as the Xenopus EF1alpha enhancer/promoter to improve vector expression in the brain. In addition, the vector is dicistronic and utilizes an IRES to allow translation of the reporter gene, human placental alkaline phosphatase (PLAP).

    How to cite this plasmid

    These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

    • For your Materials & Methods section:

      pCLE was a gift from Nicholas Gaiano (Addgene plasmid # 17703)

    • For your References section:

      Radial glial identity is promoted by Notch1 signaling in the murine forebrain. Gaiano N, Nye JS, Fishell G. Neuron. 2000 May . 26(2):395-404. 10.1016/S0896-6273(00)81172-1 PubMed 10839358