Product: Anti-DcR1 pAb
Cat. No.: PC-533 (100 μg)
Synonyms:
Anti-TRAIL-R3, anti-TRID, anti-LIT.
Background:
Apoptosis is induced by certain cytokines
including TNF and Fas ligand in the TNF family
through their death domain containing receptors.
TRAIL/Apo2L is a new member of the TNF
family and induces apoptosis of a variety of
tumor cell lines. DR4 and DR5 are the recently
identified functional receptors for TRAIL. Two
decoy receptors for TRAIL have been identified
and designated DcR1/TRID/TRAIL-R3/LIT and
DcR2/TRAIL-R4/TRUNDD. DcR1 has
extracellular TRAIL-binding domain but lacks
intracellular signaling domain. It is a
glycophospholipid-anchored cell surface protein.
DcR1 transcripts were expressed in many
normal human tissues but not in most cancer
cell lines. Over-expression of DcR1 did not
induce apoptosis, but attenuated TRAIL-induced
apoptosis.
Specificity:
Recognizes DcR1.
Positive Controls:
HeLa cell lysate (~65 kDa band detected by
Western blot).
Species Reactivity:
Human, mouse and rat. Other species not tested.
Host:
Rabbit
Immunogen:
A peptide corresponding to amino acids in an
extracellular domain (ED) of human DcR1
precursor.
Format:
100 μg of polyclonal antibody in PBS containing
0.02% sodium azide. Purified by immunoaffinity
chromatography.
Storage:
Store at 4°C short term, -20°C long term. Aliquot
to avoid repeat freeze/thaw cycles.
Applications and Suggested Dilutions:

Western blot: Use at 0.5 - 1 μg/mL.

Immunocytochemistry: Use at ~10 μg/mL.
The optimal dilution for a specific application
should be determined by the researcher.
Figure 1: Western blot analysis of DcR1 in HeLa
whole cell lysates with anti-DcR1 pAb at 1 μg/mL.
Figure 2: Immunocytochemistry of DcR1 in HeLa
cells with anti-DcR1 pAb at 10 μg/mL.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.