CASP4 polyclonal antibody
产品名称： CASP4 polyclonal antibody
英文名称： CASP4 polyclonal antibody
产品编号： PAB6681
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Goat polyclonal antibody raised against synthetic peptide of CASP4.
- Immunogen:
- A synthetic peptide corresponding to human CASP4.
- Sequence:
- CKERAEEIYPIKERN
- Host:
- Goat
- Theoretical MW (kDa):
- 43.3, 36.7
- Reactivity:
- Human
- Specificity:
- This antibody is expected to recognize all 3 isoforms of CASP4 (alpha, represented by NP_001216, gamma, NP-150649 and delta NP_150650).
- Form:
- Liquid
- Purification:
- Antigen affinity purification
- Concentration:
- 0.5 mg/mL
- Storage Buffer:
- In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
- Storage Instruction:
- Store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:16000)
Western Blot (0.3-2 ug/mL)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Molecular cloning and pro-apoptotic activity of ICErelII and ICErelIII, members of the ICE/CED-3 family of cysteine proteases.
Munday NA, Vaillancourt JP, Ali A, Casano FJ, Miller DK, Molineaux SM, Yamin TT, Yu VL, Nicholson DW.J Biol Chem. 1995 Jun 30;270(26):15870-6.
- Applications
- Western Blot (Tissue lysate)
- CASP4 polyclonal antibody (Cat # PAB6681) (0.3 ug/mL) staining of human heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Entrez GeneID:
- 837
- Protein Accession#:
- NP_001216;NP_150649;NP_150650
- Gene Name:
- CASP4
- Gene Alias:
- ICE(rel)II,ICEREL-II,ICH-2,Mih1/TX,TX
- Gene Description:
- caspase 4, apoptosis-related cysteine peptidase
- Omim ID:
- 602664
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq
- Other Designations:
- apoptotic cysteine protease Mih1/TX,caspase 4,caspase 4, apoptosis-related cysteine protease